) is an enzyme
of the ligase
class that catalyzes
(depending on the species) the physiologically irreversible carboxylation
to form oxaloacetate
Image:Pyruvic-acid-2D-skeletal.png | Pyruvic acid
Image:Oxaloacetic acid.png | Oxaloacetic acid
The reaction it catalyzes is:
pyruvate + + ATP → oxaloacetate + ADP + P
It is an important anaplerotic reaction
that creates oxaloacetate from pyruvate. The enzyme is a mitochondrial protein
containing a biotin
prosthetic group, requiring magnesium
and acetyl CoA.
Pyruvate carboxylase was first discovered in 1959 at Western Reserve University
by M. F. Utter and D. B. Keech. Since then it has been found in a wide variety of prokaryotes and eukaryotes including fungi, bacteria, plants, and animals. In mammals, PC plays a crucial role in gluconeogenesis and lipogenesis, in the biosynthesis of neurotransmitter
s, and in glucose-induced insulin secretion by pancreatic islets. Oxaloacetate produced by PC is an important intermediate, which is used in these biosynthetic pathways. In mammals, PC is expressed in a tissue-specific manner, with its activity found to be highest in the liver and kidney (gluconeogenic tissues), in adipose tissue and lactating mammary gland (lipogenic tissues), and in pancreatic islets. Activity is moderate in brain, heart and adrenal gland, and least in white blood cells and skin fibroblasts.
Structural studies of PC have been conducted by electron microscopy
, by limited proteolysis
, and by cloning and gasa sequencing of genes and cDNA encoding the enzyme. Most well characterized forms of active PC consist of four identical subunits arranged in a tetrahedron-like structure. Each subunit contains a single biotin
moiety acting as a swinging arm to transport carbon dioxide
to the catalytic site that is formed at the interface between adjacent monomers. Each subunit of the functional tetramer contains four domains: the biotin carboxylation (BC) domain, the transcarboxylation (CT) domain, the biotin carboxyl carrier (BCCP) domain and the recently termed PC tetramerization (PT) domain. From the two most complete crystal structures available, an asymmetric and symmetric form of the protein have been visualized. The Staphylococcus aureus
tetramer in complex with the activator coenzyme A is highly symmetric, possessing 222 symmetry, and has been confirmed by cryo-EM studies. In contrast the Rhizobium etli
, tetramer in complex with ethyl-CoA, a non-hydrolyzable analog of acetyl-CoA
, possesses only one line of symmetry.
Pyruvate carboxylase uses a covalently attached biotin
cofactor which is used to catalyze the ATP
– dependent carboxylation of pyruvate to oxaloacetate in two steps. Biotin is initially carboxylated at the BC active site by ATP and bicarbonate. The carboxyl group is subsequently transferred by carboxybiotin to a second active site in the CT domain, where pyruvate is carboxylated to generate oxaloacetate. The BCCP domain transfers the tethered cofactor between the two remote active sites. The allosteric binding site in PC offers a target for modifiers of activity that may be useful in the treatment of obesity or type II diabetes, and the mechanistic insights gained from the complete structural description of RePC (R. etli) permit detailed investigations into the individual catalytic and regulatory sites of the enzyme.
The reaction mechanism can be subdivided into two partial reactions (see figure to the right). In the first reaction, ATP
is carboxylated to produce carbonic phosphoric anhydride −O(−O)P(=O)O–C(=O)O−
which in turn carboxylates a biotin
cofactor that is covalently attached to a lysine residue of the BCCP domain. Carbonic phosphoric anhydride decomposes into carbon dioxide and phosphate prior to attack by the enzyme linked biotin molecule. In most species, this reaction requires acetyl-CoA
as an allosteric activator binding to the PT domain. In the second reaction, occurring in the CT domain of an adjacent monomer, carbon dioxide is transferred to the acceptor molecule, pyruvate, to form oxaloacetate. The reaction proceeds via the removal of a proton from pyruvate, by an as yet unidentified active site residue, to generate an enol
ate intermediate. The enolate intermediate then attacks CO2 transiently released from the enzyme linked biotin molecule. The resultant oxaloacetate
is released. The biotin molecule is protonated by the aforementioned active site residue and released from the active site of the CT domain to be recarboxylated. The major regulator of enzyme activity, acetyl-CoA, stimulates the cleavage of ATP in the first partial reaction and also it has been shown to induce a conformational change in the tetrameric structure of the enzyme.
, pyruvate carboxylase is involved in the synthesis of phosphoenolpyruvate
(PEP) from pyruvate
. Pyruvate is first converted by pyruvate carboxylase to oxaloacetate
(OAA) in the mitochondrion requiring hydrolysis
of one molecule of ATP
. The OAA is then decarboxylated and simultaneously phosphorylated, which is catalyzed by one of two isoforms of phosphoenolpyruvate carboxykinase
(PEPCK) either in the cytosol
or in the mitochondria
to produce PEP. Under ordinary gluconeogenic conditions, OAA is converted into PEP by mitochondrial PEPCK; the resultant PEP is then transported out of the mitochondrial matrix by an anion transporter carrier system, and converted into glucose by cytosolic gluconeogenic enzymes. However, during starvation when cytosolic NADH concentration is low and mitochrondrial NADH levels are high oxaloacetate can be used as a shuttle of reducing equivalents. As such OAA is converted into malate
by mitochondrial Malate dehydrogenase
(MDH). After export into the cytosol, malate is converted back into OAA, with concomitant reduction of NAD+; OAA is subsequently converted to PEP which is available for gluconeogenesis in the cytosol along with the transported reducing equivalent NADH.
Very high levels of PC activity, together with high activities of other gluconeogenic enzymes including PEPCK
in liver and kidney cortex, suggest that a primary role of PC is to participate in gluconeogenesis in these organs. During fasting or starvation when endogenous glucose is required for certain tissues (brain, white blood cells and kidney medulla), expression of PC and other gluconeogenic enzymes is elevated. In rats and mice, alteration of nutrition status has been shown to affect hepatic PC activity. Fasting promotes hepatic glucose production sustained by an increased pyruvate flux, and increases in PC activity and protein concentration; diabetes similarly increases gluconeogenesis through enhanced uptake of substrate and increased flux through liver PC in mice and rats. Similarly to other gluconeogenic enzymes, PC is positively regulated by glucagon
s while negatively regulated by insulin
. Further supporting the key role of PC in gluconeogenesis, in dairy cattle, which have hexose
absorption ability at adequate nutrition levels, PC and the associated gluconeogenic enzyme PEPCK are markedly elevated during the transition to lactation in proposed support of lactose synthesis for milk production.
Aside from the role of PC in gluconeogenesis, PC serves an anaplerotic
role (an enzyme catalyzed reaction that can replenish the supply of intermediates in the citric acid cycle) for the tricarboxylic acid cycle
(essential to provide oxaloacetate), when intermediates are removed for different biosynthetic purposes.
As a crossroad between carbohydrate
metabolism, pyruvate carboxylase expression in gluconeogenic tissues, adipose tissues and pancreatic islets must be coordinated. In conditions of over nutrition, PC levels are increased in pancreatic β-cells to increase pyruvate cycling
in response to chronically elevated levels of glucose
. In contrast, PC enzyme levels in the liver are decreased by insulin
; during periods of overnutrition adipocyte tissue is expanded with extreme expression of PC and other lipogenic enzymes. Hepatic control of glucose levels is still regulated in an over nutrition situation, but in obesity induced type 2 diabetes the regulation of peripheral glucose levels is no longer under regulation of insulin.
In type 2 diabetic
rats, chronic exposure of β-cells
to glucose due to peripheral insulin
resistance results in decreased PC enzyme activity and decreased pyruvate
cycling The continued overproduction of glucose by hepatocytes
causes dramatic alteration of β-cell gene
expression with large increases in normally suppressed genes, and equivalent decreases in expression of mRNA for insulin, ion pumps necessary for insulin secretion, and metabolic enzymes related to insulin secretion, including pyruvate carboxylase Concurrently adipose tissue develops insulin resistance causing accumulation of triaglycerols and non-esterified fatty acids
in circulation; these not only further impairing β-cell function, but also further decreasing PC expression. These changes result in the decline of the β-cell
phenotype in decompensated diabetes.
A deficiency of pyruvate carboxylase
can cause lactic acidosis
as a result of lactate
build up. Normally, excess pyruvate
is shunted into gluconeogenesis
via conversion of pyruvate into oxaloacetate
, but because of the enzyme deficiency, excess pyruvate is converted into lactate
instead. As a key role of gluconeogenesis is in the maintenance of blood sugar
, deficiency of pyruvate carboxylase can also lead to hypoglycemia